murine origin  (Santa Cruz Biotechnology)

Journal: Polymers

Article Title: Ultrasound-Assisted Extraction of Adenophora triphylla Polysaccharides: Optimization and Characterization of Physicochemical and Functional Properties

doi: 10.3390/polym18040457

Figure Lengend Snippet: Anti-inflammatory activity of WE-ATRPs and UAE-ATRPs in LPS-induced activated RAW264.7 cells. RAW264.7 cells were pretreated with ATPRs for 1 h, then cotreated with or without LPS (1 μg/mL) for 24 h. Nitric oxide synthesis was measured by the Griess reagent ( A ). Cell viability was measured using the CCK-8 assay ( B ). WE-ATRPs = ATRPs extracted by water; UAE-ATRPs = ATRPs extracted by ultrasound-assisted extraction. The data are presented as mean ± SD. If the measurement value was too low or not observed, it is marked as N.D. (Not detected). # p < 0.01 comparing LPS with the non-treatment group (NC). ** p < 0.01 comparing the experimental group with the LPS-only treatment group (LPS). ‘ns’ refers to ‘not significant’ comparing non-treatment with the experimental group.

Article Snippet: Murine origin macrophage cell line RAW264.7 cells were purchased from the Korean cell line bank (Seoul, Republic of Korea), and cultured in Dulbecco’s modified Eagle medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin under a humidified atmosphere at 5% CO 2 and 37 °C.

Techniques: Activity Assay, CCK-8 Assay, Extraction

Journal: Foods

Article Title: Effects of Protein Hydrolysate from Silkworm ( Bombyx mori ) pupae on the C2C12 Myogenic Differentiation

doi: 10.3390/foods12152840

Figure Lengend Snippet: Effect of protein hydrolysate from silkworm pupae (SP) on C2C12 myogenic differentiation: immunofluorescence assay. ( A ) C2C12 cells immunostained with anti-MyHC and DAPI were treated with different concentrations of SP (50, 100, 200, and 400 μg/mL); ( B ) the effect of SP on the fusion index for myotube formation; and ( C ) the My-HC positive cells were classified to mononucleus, 2–5 nuclei, and >5 nuclei. Three independent data were expressed as mean ± SD. Different superscripts indicate a significant difference ( p < 0.05).

Article Snippet: Murine-origin C2C12 (ATCC, Manassas, VA, USA) myoblast cells were seeded in a 10 cm cell culture dish at a cell density of 3 × 10 5 .

Techniques: Immunofluorescence

Journal: Foods

Article Title: Effects of Protein Hydrolysate from Silkworm ( Bombyx mori ) pupae on the C2C12 Myogenic Differentiation

doi: 10.3390/foods12152840

Figure Lengend Snippet: Effect of fractionated protein hydrolysate from silkworm pupae (SP-Fs) on C2C12 myogenic differentiation: immunofluorescence assay. C2C12 cells immunostained with anti-MyHC and DAPI were treated with different concentrations of SP-Fs (50, 100, 200, and 400 μg/mL). ( A ), control; ( B ), SP-F1; ( C ), SP-F2; ( D ), SP-F3; ( E ), SP-F4; and ( F ), the effect of SP-Fs on the fusion index for myotube formation. Three independent data were expressed as mean ± SD. Different superscripts indicate a significant difference ( p < 0.05).

Article Snippet: Murine-origin C2C12 (ATCC, Manassas, VA, USA) myoblast cells were seeded in a 10 cm cell culture dish at a cell density of 3 × 10 5 .

Techniques: Immunofluorescence, Control

Journal: Foods

Article Title: Effects of Protein Hydrolysate from Silkworm ( Bombyx mori ) pupae on the C2C12 Myogenic Differentiation

doi: 10.3390/foods12152840

Figure Lengend Snippet: Effects of the subfraction 1 of silkworm pupae protein hydrolysate (SP-F1) on the expression of MyoD, myogenin, and MyHC of C2C12 myogenic differentiation. ( A ) Western blot analyses were performed to examine the expression of the myogenic regulatory factor proteins, namely MyoD ( B ), Myogenin ( C ), and MyHC ( D ), and their levels were quantified. Three independent data were expressed as mean ± SD. Different superscripts indicate a significant difference ( p < 0.05).

Article Snippet: Murine-origin C2C12 (ATCC, Manassas, VA, USA) myoblast cells were seeded in a 10 cm cell culture dish at a cell density of 3 × 10 5 .

Techniques: Expressing, Western Blot